Proteomic and Transcriptomic Analyses Provide Novel Insights into the Crucial Roles of Host-Induced Carbohydrate Metabolism Enzymes in Xanthomonas oryzae pv. oryzae Virulence and Rice-Xoo Interaction

作者：Guichun Wu, Yuqiang Zhang, Bo Wang, Kaihuai Li, Yuanlai Lou, Yancun Zhao, Fengquan Liu · 发表于：Rice · 年份：2021 · DOI：10.1186/s12284-021-00503-x · 被引用次数：20 · 研究领域：Plant Pathogenic Bacteria Studies、Plant-Microbe Interactions and Immunity、Plant pathogens and resistance mechanisms

Abstract Background Xanthomonas oryzae pv. oryzae ( Xoo ) causes bacterial leaf blight, a devastating rice disease. The Xoo -rice interaction, wherein wide ranging host- and pathogen-derived proteins and genes wage molecular arms race, is a research hotspot. Hence, the identification of novel rice-induced Xoo virulence factors and characterization of their roles affecting rice global gene expression profiles will provide an integrated and better understanding of Xoo -rice interactions from the molecular perspective. Results Using comparative proteomics and an in vitro interaction system, we revealed that 5 protein spots from Xoo exhibited significantly different expression patterns (|fold change| > 1.5) at 3, 6, 12 h after susceptible rice leaf extract (RLX) treatment. MALDI-TOF MS analysis and pathogenicity tests showed that 4 host-induced proteins, including phosphohexose mutase, inositol monophosphatase, arginase and septum site-determining protein, affected Xoo virulence. Among them, mutants of two host-induced carbohydrate metabolism enzyme-encoding genes, Δ xanA and Δ imp , elicited enhanced defense responses and nearly abolished Xoo virulence in rice. To decipher rice differentially expressed genes (DEGs) associated with xanA and imp , transcriptomic responses of Δ xanA -treated and Δ imp -treated susceptible rice were compared to those in rice treated with PXO99 A at 1 and 3 dpi. A total of 1521 and 227 DEGs were identified for PXO99 A vs Δ imp at 1 and 3 dpi, whil...