Cotreatment with Aspirin and Azole Drugs Increases Sensitivity of Candida albicans in vitro

作者：Wenli Feng, Jing Yang, Yan Ma, Zhiqin Xi, Ying Ji, Qiao Ren, Huan Ning, Shaoyan Wang · 发表于：Infection and Drug Resistance · 年份：2021 · DOI：10.2147/idr.s314538 · 被引用次数：8 · 研究领域：Antifungal resistance and susceptibility、Antimicrobial Resistance in Staphylococcus、Pneumonia and Respiratory Infections

Purpose: This study aimed to investigate the effects of aspirin (acetyl salicylic acid [ASA]) combined with fluconazole (FCA), itraconazole (ITR), or voriconazole (VRC) on Candida albicans under planktonic and biofilm conditions. Methods: A total of 39 clinical C. albicans strains were used to perform the in vitro drug sensitivity assay under different conditions using the M27-A4 broth microdilution method. The minimal inhibitory concentrations (MICs) and fractional inhibitory concentration index (FICI) values were calculated. C. albicans ZY23 was chosen for the further analyses. Results: Under planktonic conditions, the half maximal MIC (MIC 50 ) values of FCA, ITR, and VRC were 64– 0.5 μg/mL, 32– 0.0625 μg/mL, and 16– 0.125 μg/mL, respectively, when applied, whereas in combination with ASA, the values decreased to 32– 0.25 μg/mL, 8– 0.0313 μg/mL, and 8– 0.0313 μg/mL, respectively. Under biofilm conditions, FCA, ITR, or VRC alone showed MIC 50 values of 128– 8 μg/mL, 32– 4 μg/mL, and 32– 0.5 μg/mL, whereas in combination with ASA the values were decreased to 32– 0.5 μg/mL, 16– 0.5 μg/mL, and 8– 0.0625 μg/mL, respectively. Analysis of the FICI showed that the sensitization rate of ASA to FCA, ITR, and FCA under planktonic conditions was 43.59%, whereas the sensitization rates of ASP to FCA, ITR, and FCA under biofilm conditions were 46.15%, 46.15%, and 48.72%, respectively. Additionally, the time-growth and time-kill curves of C. albicans ZY23 further verified the synergistic...