Unilateral cervical spinal cord injury induces bone loss and metabolic changes in non-human primates (Macaca fascicularis)

作者：Xiuhua Wu, Xiaolin Xu, Qi Liu, Jianyang Ding, Junhao Liu, Zhiping Huang, Zucheng Huang, Xiaoliang Wu, Rong Li, Yang Zhou, Hui Jiang, Jie Liu, Qingàn Zhu · 发表于：Journal of Orthopaedic Translation · 年份：2021 · DOI：10.1016/j.jot.2021.03.006 · 被引用次数：11 · 研究领域：Spinal Cord Injury Research、Cervical and Thoracic Myelopathy、Nerve injury and regeneration

BACKGROUND/OBJECTIVE: The deleterious effects of chronic spinal cord injury (SCI) on the skeleton in rats, especially the lower extremities, has been proved previously. However, the long-term skeletal changes after SCI in non-human primates (NHP) have been scarcely studied. This study aimed to evaluate the bone loss in limbs and vertebrae and the bone metabolic changes in NHP after unilateral cervical spinal cord contusion injury. METHODS: were randomly divided into the SCI (n=8) and the Sham (n=4) groups. The SCI models were established using hemi-contusion cervical spinal cord injury on fifth cervical vertebra (C5), and were further evaluated by histological staining and neurophysiological monitoring. Changes of bone microstructures, bone biomechanics, and bone metabolism markers were assessed by micro-CT, micro-FEA and serological kit. RESULTS: The NHP hemi-contusion cervical SCI model led to consistent unilateral limb dysfunction and potential plasticity in the face of loss of spinal cord. Furthermore, the cancellous bone mass of ipsilateral humerus and radius decreased significantly compared to the contralateral side. The bone volume fraction of humerus and radius were 17.2% and 20.1% on the ipsilateral while 29.0% and 30.1% on the contralateral respectively. Similarly, the thickness of the cortical bone in the ipsilateral forelimbs was significantly decreased, as well as the bone strength of the ipsilateral forelimbs. These changes were accompanied by diminished concent...