Genetically Encoded, pH-Sensitive mTFP1 Biosensor for Probing Lysosomal pH

作者：Marcus Y. Chin, Anand R. Patwardhan, Kean-Hooi Ang, Austin L. Wang, Carolina Alquézar, Mackenzie Welch, Phi T. Nguyen, Michael Grabe, Anna Victoria Molofsky, Michelle R. Arkin, Aimee W. Kao · 发表于：ACS Sensors · 年份：2021 · DOI：10.1021/acssensors.0c02318 · 被引用次数：95 · 研究领域：Advanced Fluorescence Microscopy Techniques、Cellular transport and secretion、Calcium signaling and nucleotide metabolism

of 4.3. Ratiometric quantification was enabled with cytosolically oriented mCherry using high-content quantitative imaging. We expressed FIRE-pHLy in several cellular models and quantified the alkalinizing response to bafilomycin A1, a specific V-ATPase inhibitor. In summary, we have engineered FIRE-pHLy, a specific, robust, and versatile lysosomal pH biosensor, that has broad applications for investigating pH dynamics in aging- and lysosome-related diseases, as well as in lysosome-based drug discovery.