Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

作者：Wenbin Liu, Min-meng Wang, Liuye Dai, Shenghua Dong, Xiudan Yuan, Shuli Yuan, Yi Tang, Jinhui Liu, Liangyue Peng, Yamei Xiao · 发表于：Frontiers in Physiology · 年份：2021 · DOI：10.3389/fphys.2021.666363 · 被引用次数：18 · 研究领域：Aquaculture disease management and microbiota、Aquaculture Nutrition and Growth、Reproductive biology and impacts on aquatic species

. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stress. Exposure to 9 mg/L Cd for 96 h changed the hepatic function indexes remarkably in the 2n fish, but not in the 3n fish. In the serum of Cd-treated 2n fish, the levels of alanine amino transferase, aspartate aminotransferase, adenosine deaminase, and total bilirubin significantly increased, while the levels of total protein, albumin, lysozyme, and anti-superoxide anion radicals decreased demonstrating hepatotoxicity. By analysis of transcriptome profiles, many immune-related pathways were found to be involved in the response of 3n fish to the Cd-induced stress. Expression levels of the immune genes, including the interleukin genes, tumor necrosis factor super family member genes, chemokine gene, toll-like receptor gene, and inflammatory marker cyclooxygenase 2 gene were significantly enhanced in the hepatopancreas of the Cd-treated 3n fish. In contrast, the expression levels of these genes decreased in the 2n fish. This research provides a theoretical basis for polyploid fish breeding and is helpful for the ecological restoration of water due to pollution.