Near-infrared photoactive Yb-MOF functionalized with a large conjugate ionic liquid: synthesis and application for photoelectrochemical immunosensing of carcinoma embryonic antigen

作者：Huiyue Li, Ying Li, Xue Zhang, Pan Liu, Meng He, Chunya Li, Yanying Wang · 发表于：Nanoscale · 年份：2021 · DOI：10.1039/d1nr01606g · 被引用次数：30 · 研究领域：Advanced biosensing and bioanalysis techniques、Advanced Photocatalysis Techniques、Nanoplatforms for cancer theranostics

A novel near-infrared (NIR)-excited photoelectrochemical (PEC) immunosensor based on an ionic liquid functionalized metal organic framework (Yb-MOF) and gold nanoparticles (Au-NPs) was designed for the high-performance determination of carcinoembryonic antigen (CEA). The Yb-MOF was synthesized from the coordination of the Yb3+ metal ion with the 1,1'-(1,5-dihydropyrene-2,7-diyl)bis(3-(4-carboxybenzyl)-1H-imidazol-3-ium) bromide [DDPDBCBIm(Br)2] ionic liquid by a hydrothermal method. To improve the photoelectric conversion efficiency of the Yb-MOF in the NIR region, the surface of the Yb-MOF was integrated with gold nanoparticles (AuNPs) to fabricate a Yb-MOF@AuNP nanocomposite through an in situ reduction of chloroauric acid with sodium borohydride. The NIR photoelectrochemical response of the Yb-MOF@AuNPs at 808 nm was enhanced 4-fold over the pristine Yb-MOF. Subsequently, a photoelectrochemical platform based on the Yb-MOF@AuNPs was constructed for loading the CEA antibody (anti-CEA). After cross-linking with glutaraldehyde followed by blocking with bovine serum albumin, a photoelectrochemical sensor for assaying CEA was fabricated. Upon specifically interacting with CEA, CEA can block the photogenerated electron-hole pair transfer and the mass transfer of ascorbic acid to the sensing interface, thus leading to a decrease in photocurrent response. The photocurrent variation can be used for determining CEA quantitatively. After optimizing the experimental conditions, the ph...