DNA-methylation-based telomere length estimator: comparisons with measurements from flow FISH and qPCR

作者：Emily Pearce, Steve Horvath, Shilpa Katta, Casey Dagnall, Geraldine Aubert, Belynda Hicks, Stephen R. Spellman, Hormuzd A. Katki, Sharon A. Savage, Rotana Alsaggaf, Shahinaz M. Gadalla · 发表于：Aging · 年份：2021 · DOI：10.18632/aging.203126 · 被引用次数：36 · 研究领域：Telomeres, Telomerase, and Senescence、Cytomegalovirus and herpesvirus research、Single-cell and spatial transcriptomics

< 0.0001). The agreements between flow FISH TL and DNAmTL or qPCR were acceptable but with wide limits of agreement. DNAmTL correctly classified >70% of TL categorized above or below the median, but the accuracy dropped with increasing TL categories. The ability of DNAmTL to detect associations with age and other TL-related factors in the absence of strong correlation with measured TL may indicate its capture of aspects of telomere maintenance mechanisms and not necessarily TL. The inaccuracy of DNAmTL prediction should be considered during data interpretation and across-study comparisons.