The protective effects of exogenous spermine on renal ischemia-reperfusion injury in rats

作者：Bo Yan, Shaoju Min, Bin Xu, Cheng Zhang, Jun Pei, Wei Zhang, Guangheng Luo · 发表于：Translational Andrology and Urology · 年份：2021 · DOI：10.21037/tau-21-280 · 被引用次数：12 · 研究领域：Polyamine Metabolism and Applications、Aldose Reductase and Taurine、Protein Hydrolysis and Bioactive Peptides

BACKGROUND: To investigate the protective effects of exogenous spermine on renal ischemia-reperfusion injury in rats. METHODS: (I) Different doses of spermine were injected into rats to determine the safe dose on the kidneys. Kidney toxicity was assessed by hematoxylin and eosin (HE) staining of kidney tissue and enzyme-linked immunosorbent assay (ELISA) detection of neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule 1 (KIM-1) in the venous blood. (II) A rat model of renal ischemia-reperfusion injury was established. Different doses of spermine were injected into the rats through the tail vein 30 minutes before and 3 days after the establishment of the model. Blood samples and kidney tissues were collected and renal injury was assessed via HE staining of the renal tissue, detection of apoptosis using the TUNEL assay, and detection of NGAL and KIM-1 in blood samples using ELISA. (III) Human HK-2 renal tubular epithelial cells were cultured under hypoxia/reoxygenation conditions. To evaluate the protective effects of spermine, apoptosis was assessed by flow cytometry and TUNEL assay. The mechanisms underlying the effects of spermine were studied using Western blot analyses. RESULTS: At spermine concentrations below 200 µM (2 mL/kg body weight), no significant damage to the kidney was observed by HE staining, and there was no significant difference in NGAL and KIM-1 levels between rats treated with spermine and control rats (P<0.05). At spermine doses b...