Large-scale analysis of 2,152 Ig-seq datasets reveals key features of B cell biology and the antibody repertoire

作者：Xiujia Yang, Minhui Wang, Jiaqi Wu, Dianchun Shi, Yanfang Zhang, Huikun Zeng, Yan Zhu, Chunhong Lan, Yang Deng, Shixin Guo, Lijun Xu, Cuiyu Ma, Yanxia Zhang, Jinxia Ou, Chu‐Jun Liu, Yuan Chen, Qilong Wang, Wenxi Xie, Junjie Guan, Jieyu Ding, Zhi Wang, Changqing Chang, Wei Yang, Huijie Zhang, Jun Chen, Lijie Qin, Hongwei Zhou, Jin‐Xin Bei, Lai Wei, Guangwen Cao, Xueqing Yu, Zhenhai Zhang · 发表于：Cell Reports · 年份：2021 · DOI：10.1016/j.celrep.2021.109110 · 被引用次数：23 · 研究领域：T-cell and B-cell Immunology、Monoclonal and Polyclonal Antibodies Research、Immune Cell Function and Interaction

Antibody repertoire sequencing enables researchers to acquire millions of B cell receptors and investigate these molecules at the single-nucleotide level. This power and resolution in studying humoral responses have led to its wide applications. However, most of these studies were conducted with a limited number of samples. Given the extraordinary diversity, assessment of these key features with a large sample set is demanded. Thus, we collect and systematically analyze 2,152 high-quality heavy-chain antibody repertoires. Our study reveals that 52 core variable genes universally contribute to more than 99% of each individual's repertoire; a distal interspersed preferences characterize V gene recombination; the number of public clones between two repertoires follows a linear model, and the positive selection dominates at RGYW motif in somatic hypermutations. Thus, this population-level analysis resolves some critical features of the antibody repertoire and may have significant value to the large cadre of scientists.