MicroRNA panel in serum reveals novel diagnostic biomarkers for prostate cancer

作者：Shiyu Zhang, Cheng Liu, Xuan Zou, Xiangnan Geng, Xin Zhou, X. Fan, Danxia Zhu, Huo Zhang, Wei Zhu · 发表于：PeerJ · 年份：2021 · DOI：10.7717/peerj.11441 · 被引用次数：25 · 研究领域：MicroRNA in disease regulation、Extracellular vesicles in disease、Cancer-related molecular mechanisms research

PURPOSE: MicroRNAs (miRNAs), which could be stably preserved and detected in serum or plasma, could act as biomarkers in cancer diagnosis. Prostate cancer is the second cancer in males for incidence. This study aimed to establish a miRNA panel in peripheral serum which could act as a non-invasive biomarker helping diagnosing PC. METHODS: A total of 86 PC patients and 86 normal control serum samples were analyzed through a four-stage experimental process using quantitative real-time polymerase chain reaction. Logistic regression method was used to construct a diagnostic model based on the differentially expressed miRNAs in serum. Receiver operating characteristic curves were constructed to evaluate the diagnostic accuracy. We also compared the 3-miRNA panel with previously reported biomarkers and verified in four public datasets. In addition, the expression characteristics of the identified miRNAs were further explored in tissue and serum exosomes samples. RESULTS: We identified a 3-miRNA signature including up-regulated miR-146a-5p, miR-24-3p and miR-93-5p for PC detection. Areas under the receiver operating characteristic curve of the 3-miRNA panel for the training, testing and external validation phase were 0.819, 0.831 and 0.814, respectively. The identified signature has a very stable diagnostic performance in the large cohorts of four public datasets. Compared with previously identified miRNA biomarkers, the 3-miRNA signature in this study has superior performance in dia...