Profiling Humoral Immune Response Against Pre-Erythrocytic and Erythrocytic Antigens of Malaria Parasites Among Neotropical Primates in the Brazilian Atlantic Forest

作者：Gabriela Maíra Pereira de Assis, Denise Anete Madureira de Alvarenga, Matheus de Oliveira Costa Pereira, Juan Camilo Sánchez‐Arcila, Anielle de Pina Costa, Júlio César de Souza, Ana Júlia Dutra Nunes, Alcides Pissinatti, Sílvia Bahadian Moreira, Letícia de Menezes Torres, Helena L. Costa, Herlandes da Penha Tinoco, Valéria do Socorro Pereira, Irene S. Soares, Taís Nóbrega de Sousa, Francis B. Ntumngia, John H. Adams, Flora Satiko Kano, Zelinda Maria Braga Hirano, Cláudio Tadeu Daniel‐Ribeiro, Joseli Oliveira Ferreira, Luzia H. Carvalho, Cristiana Ferreira Alves de Brito · 发表于：Frontiers in Cellular and Infection Microbiology · 年份：2021 · DOI：10.3389/fcimb.2021.678996 · 被引用次数：12 · 研究领域：Malaria Research and Control、Mosquito-borne diseases and control、HIV Research and Treatment

Human malaria due to zoonotic transmission has been recorded in the Atlantic Forest, an extra-Amazonian area in Brazil, which are a challenge for malaria control. Naturally acquired humoral immune response against pre-erythrocytic and erythrocytic antigens of Neotropical primates (NP) was evaluated here to improve the knowledge about the exposure of those animals to the malaria transmission and support the identification of the potential reservoirs of the disease in the Atlantic Forest. Blood samples of 154 monkeys from three areas of the Atlantic Forest were used to identify IgG antibodies against peptides of the repeat region of the major pre-erythrocytic antigen, the circumsporozoite protein (CSP), of Plasmodium vivax (PvCSP), Plasmodium brasilianum/Plasmodium malariae (Pb/PmCSP), and Plasmodium falciparum (PfCSP) by ELISA. Antibodies against erythrocytic recombinant antigens of P. vivax , Apical membrane antigen 1 (PvAMA-1), Erythrocyte binding protein 2 (PvEBP-2) and domain II of Duffy binding protein (PvDBPII) were also evaluated. Parameters, such as age, sex, PCR positivity, and captivity, potentially associated with humoral immune response were analyzed. Eighty-five percent of NP had antibodies against at least one CSP peptide, and 76% against at least one P. vivax erythrocytic antigen. A high percentage of adults compared to non-adults were seropositive and showed increased antibody levels. Neotropical primates with PCR positive for P. simium had a significantly high...