Nakamurella Leprariae Sp. Nov., Isolated From a Lichen Sample

作者：De-Feng An, Shao-Juan Yang, Long-Qian Jiang, Xinyu Wang, Xiaoyu Huang, Lei Lang, Chen Xue-mei, Ming-Qun Fan, Guiding Li, Mingguo Jiang, Li‐Song Wang, Chenglin Jiang, Yi Jiang · 发表于：Research Square · 年份：2021 · DOI：10.21203/rs.3.rs-484462/v1 · 被引用次数：2 · 研究领域：Lichen and fungal ecology、Plant Pathogens and Fungal Diseases、Mycorrhizal Fungi and Plant Interactions

Abstract A novel actinobacterium, YIM 132084 T , was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China and identified by a polyphasic taxonomic approach. The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonies were round, convex, smooth and light orange yellow in colour. It grew at 10–40 ℃ (optimum 28 ℃), at pH 6.0–11.0 (optimum pH 7.0) and in the presence of 0–4 % NaCl (optimum 0 %). Strain YIM 132084 T comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major polar lipids, MK-8(H 4 ) as the predominant menaquinone, and anteiso -C 15:0 , anteiso -C 17:0 , iso -C 15:0 and iso -C 16:0 as major fatty acids. Strain YIM 132084 T had meso -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and mannose, ribose, glucose and rhamnose as whole-cell sugars. The 16S rRNA gene sequence showed high level of similarity with Nakamurella flavida KCTC 19127 T (97. 7%) and Nakamurella flava CGMCC 4.7524 T (97.7%). The G + C content of the genomic DNA was 72.4 mol%. Strain YIM 132084 T showed an average nucleotide identity value of 76.1 % and 74.9 %, a digital DNA-DNA hybridizations value of 20.9 % and 20.6 % with the reference strains Nakamurella flavida and Nakamurella flava based on draft genome sequences, respectively. The results of the phenotypic, chemotaxonomic and phylogenetic analyses, showed that strain YIM 132084 T...