Distinct Microbial Signatures between Periodontal Profile Classes

作者：Julie T. Marchesan, Kevin Moss, Thiago Morelli, Flavia Teles, Kimon Divaris, Maya Styner, Apoena Aguiar Ribeiro, Jennifer Webster‐Cyriaque, James D. Beck · 发表于：Journal of Dental Research · 年份：2021 · DOI：10.1177/00220345211009767 · 被引用次数：25 · 研究领域：Oral microbiology and periodontitis research、Dental Radiography and Imaging、HIV/AIDS oral health manifestations

Precise classification of periodontal disease has been the objective of concerted efforts and has led to the introduction of new consensus-based and data-driven classifications. The purpose of this study was to characterize the microbiological signatures of a latent class analysis (LCA)–derived periodontal stratification system, the Periodontal Profile Class (PPC) taxonomy. We used demographic, microbial (subgingival biofilm composition), and immunological data (serum IgG antibody levels, obtained with checkerboard immunoblotting technique) for 1,450 adult participants of the Dental Atherosclerosis Risk in Communities (ARIC) study, with already generated PPC classifications. Analyses relied on t tests and generalized linear models with Bonferroni correction. Men and African Americans had higher systemic antibody levels against most microorganisms compared to women and Caucasians ( P < 0.05). Healthy individuals (PPC-I) had low levels of biofilm bacteria and serum IgG levels against most periodontal pathogens ( P < 0.05). Subjects with mild to moderate disease (PPC-II to PPC-III) showed mild/moderate colonization of multiple biofilm pathogens. Individuals with severe disease (PPC-IV) had moderate/high levels of biofilm pathogens and antibody levels for orange/red complexes. High gingival index individuals (PPC-V) showed moderate/high levels of biofilm Campylobacter rectus and Aggregatibacter actinomycetemcomitans. Biofilm composition in individuals with reduced periodont...