Bulked segregant CGT‐Seq‐facilitated map‐based cloning of a powdery mildew resistance gene originating from wild emmer wheat (Triticum dicoccoides)

作者：Qiuhong Wu, Fei Zhao, Yongxing Chen, Panpan Zhang, Huaizhi Zhang, Guanghao Guo, Jingzhong Xie, Lingli Dong, Ping Lu, Miaomiao Li, Shengwei Ma, Tzion Fahima, Eviatar Nevo, Hongjie Li, Yijing Zhang, Zhiyong Liu · 发表于：Plant Biotechnology Journal · 年份：2021 · DOI：10.1111/pbi.13609 · 被引用次数：32 · 研究领域：Wheat and Barley Genetics and Pathology、Plant Disease Resistance and Genetics、Plant Pathogens and Resistance

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a widely occurring foliar disease of wheat worldwide. Wild emmer wheat (WEW, Triticum dicoccoides) (AABB, 2n = 4x = 28), the progenitor of the cultivated tetraploid and hexaploid wheat, is highly resistant to powdery mildew, and many resistance alleles were identified in this wild species. However, only Pm41 that encodes a nucleotide-binding leucine-rich repeat (NLR) protein has been cloned using a map-based cloning approach (Li et al., 2020). NLR proteins account for the major gene families of disease resistance genes (Kourelis and van der Hoorn, 2018) and are subjected to epigenetic regulation including histone acetylation and DNA methylation (Li et al., 2019). Only 31%–34% of wheat NLRs were shared across all genomes, and the numbers of unique NLR varied from 22 to 192 per wheat cultivar (Walkowiak et al., 2020). These presence–absence variations (PAVs) of NLRs are obstacles for isolating disease resistance genes based on reference genome sequences. Bulked segregant analysis (BSA), an approach for identifying genetic variations associated with disease resistance genes, has been proven effective in mapping and cloning wheat Pm5e gene in combination with next-generation sequencing (NGS) technology (Xie et al., 2020). The combination of BSA and core genome targeted sequencing (CGT-Seq), an approach to capture the major types of histone modification in the genome (Qi et al., 2018), might be used as an effecti...