lncRNA Mirt2 upregulates miR‐1246 through methylation to suppress LPS‐induced lung cell apoptosis

作者：Xuwen Xu, Yuyuan Xu, Xin Tao, Guangyu Liang · 发表于：Immunity Inflammation and Disease · 年份：2021 · DOI：10.1002/iid3.422 · 被引用次数：17 · 研究领域：Cancer-related molecular mechanisms research、Immune Response and Inflammation、Circular RNAs in diseases

INTRODUCTION: Long noncoding RNA Mirt2 has been proven to be a suppressor of lipopolysaccharide (LPS) (a key player in sepsis)-induced inflammation responses. Therefore, Mirt2 may also participate in sepsis. This study was carried out to analyze the interactions between Mirt2 and microRNA-1246 (miR-1246) in sepsis, with a specific focus on sepsis-induced acute lung injury (sepsis-ALI). METHODS: Forty sepsis patients (sepsis group; 23 males and 17 females; 40-65 years, 48.6 ± 6.3 years), 40 sepsis patients with acute lung injury (sepsis-ALI group, 23 males and 17 females; 40-65 years, 48.7 ± 6.4 years), and 40 healthy controls (control group, 23 males and 17 females; 40-65 years, 48.6 ± 6.1 years) were included. Mirt2 and miR-1246 expression in plasma samples from these patients were determined by a reverse transcription-quantitative polymerase chain reaction (PCR). Overexpression of Mirt2 and miR-1246 was achieved in human bronchial epithelial cells (HBEpCs) to explore the interaction between them. The effects of Mirt2 overexpression on miR-1246 methylation were analyzed by methylation-specific PCR. Cell apoptosis analysis was performed to analyze the role of Mirt2 and miR-1246 in the apoptosis of HBEpCs. RESULTS: Mirt2 expression was downregulated in sepsis and was further downregulated in patients with sepsis-ALI. Mirt2 and miR-1246 found to be positively correlated. Downregulation of Mirt2 and miR-1246 was observed in HBEpCs with LPS treatment. In HBEpCs, Mirt2 overexpress...