Long Noncoding RNA SNHG1 Knockdown Ameliorates Apoptosis, Oxidative Stress and Inflammation in Models of Parkinson’s Disease by Inhibiting the miR-125b-5p/MAPK1 Axis

作者：Xiao Xiao, Zhiwen Tan, Min Jia, Xiaoli Zhou, Kemei Wu, Yanbing Ding, Wenjing Li · 发表于：Neuropsychiatric Disease and Treatment · 年份：2021 · DOI：10.2147/ndt.s286778 · 被引用次数：36 · 研究领域：Cancer-related molecular mechanisms research、RNA regulation and disease、MicroRNA in disease regulation

Background: Parkinson’s disease (PD) is a prevalent neurodegenerative disease. Long noncoding RNA small molecule RNA host gene 1 (SNHG1) has been reported to play critical roles in Parkinson’s disease (PD) progression. The study aimed to further elucidate the mechanism of SNHG1 in PD pathogenesis. Methods: The levels of SNHG1, miR-125b-5p and mitogen-activated protein kinase 1 (MAPK1) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot. Cell viability and apoptosis were evaluated by Cell Counting Kit-8 (CCK-8) assay and flow cytometry, respectively. The activity of Caspase-3 or Caspase-9 was measured using a Caspase-3 or Caspase-9 Assay Kit. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, lactic dehydrogenase (LDH) activity, reactive oxygen species (ROS) generation and superoxide dismutase (SOD) activity were gauged by enzyme-linked immunosorbent assay (ELISA). Dual-luciferase reporter assay was performed to identify the relationship between miR-125b-5p and SNHG1 or MAPK1. The MPTP-induced PD mouse was used as an in vivo model of PD and MPP + -treated SK-N-SH and MN9D cells were used as in vitro models of PD. Results: SNHG1 and MAPK1 were significantly up-regulated while miR-125b-5p was down-regulated in the MPTP-induced PD mouse model and MPP + -induced PD cell models. SNHG1 silence or miR-125b-5p overexpression protected against MPP + -evoked apoptosis, oxidative stress and inflammation in SK-N-SH and MN9...