miR-21-5p targets SKP2 to reduce osteoclastogenesis in a mouse model of osteoporosis

作者：Yizhen Huang, Yute Yang, Jianle Wang, Shasha Yao, Yao Teng, Yining Xu, Zizheng Chen, Putao Yuan, Jun Gao, Shuying Shen, Jianjun Ma · 发表于：Journal of Biological Chemistry · 年份：2021 · DOI：10.1016/j.jbc.2021.100617 · 被引用次数：34 · 研究领域：Bone Metabolism and Diseases、MicroRNA in disease regulation、Circular RNAs in diseases

Osteoporosis results from an imbalance between bone formation and bone resorption. Traditional drugs for treating osteoporosis are associated with serious side effects, and thus, new treatment methods are required. This study investigated the role of differentially expressed microRNAs during osteoclast differentiation and osteoclast activity during osteoarthritis as well as the associated underlying mechanisms. We used a microarray to screen microRNAs that decreased in the process of osteoclast differentiation and verified miR-21-5p to decrease significantly using RT-qPCR. In follow-up experiments, we found that miR-21-5p targets SKP2 to regulate osteoclast differentiation. In vivo, ovariectomized mice were used to simulate perimenopausal osteoporosis induced by estrogen deficiency, and miR-21-5p treatment inhibited bone resorption and maintained bone cortex and trabecular structure. These results suggest that miR-21-5p is a new therapeutic target for osteoporosis.