High-Level Aminoglycoside Resistance in Human Clinical Klebsiella pneumoniae Complex Isolates and Characteristics of armA-Carrying IncHI5 Plasmids

作者：Xueya Zhang, Qiaoling Li, Hailong Lin, Wangxiao Zhou, Changrui Qian, Zhewei Sun, Li Lin, Hongmao Liu, Junwan Lu, Xi Lin, Kewei Li, Teng Xu, Hailin Zhang, Changchong Li, Qiyu Bao · 发表于：Frontiers in Microbiology · 年份：2021 · DOI：10.3389/fmicb.2021.636396 · 被引用次数：23 · 研究领域：Antibiotic Resistance in Bacteria、Bacteriophages and microbial interactions、Infections and bacterial resistance

Aminoglycosides are important options for treating life-threatening infections. However, high levels of aminoglycoside resistance (HLAR) among Klebsiella pneumoniae isolates have been observed to be increasing frequently. In this study, a total of 292 isolates of the K. pneumoniae complex from a teaching hospital in China were analyzed. Among these isolates, the percentage of HLAR strains was 13.7% (40/292), and 15 aminoglycoside resistance genes were identified among the HLAR strains, with rmtB being the most dominant resistance gene (70%, 28/40). We also described an armA -carrying Klebsiella variicola strain KP2757 that exhibited a high-level resistance to all aminoglycosides tested. Whole-genome sequencing of KP2757 demonstrated that the strain contained one chromosome and three plasmids, with all the aminoglycoside resistance genes (including two copies of armA and six AME genes) being located on a conjugative plasmid, p2757-346, belonging to type IncHI5. Comparative genomic analysis of eight IncHI5 plasmids showed that six of them carried two copies of the intact armA gene in the complete or truncated Tn 1548 transposon. To the best of our knowledge, for the first time, we observed that two copies of armA together with six AME genes coexisted on the same plasmid in a strain of K. variicola with HLAR. Comparative genomic analysis of eight armA -carrying IncHI5 plasmids isolated from humans and sediment was performed, suggesting the potential for dissemination of these pl...