Effect of resveratrol on mouse ovarian vitrification and transplantation

作者：Dalin Wang, Menghui Geng, Dongying Gan, Gege Han, Gao Gao, Aying Xing, Yugui Cui, Yanqiu Hu · 发表于：Reproductive Biology and Endocrinology · 年份：2021 · DOI：10.1186/s12958-021-00735-y · 被引用次数：36 · 研究领域：Reproductive Biology and Fertility、Sirtuins and Resveratrol in Medicine、Ovarian function and disorders

Abstract Background After ovarian tissue transplantation, ischemia-reperfusion injury and free radicals cause follicle depletion and apoptosis. Therefore, the use of antioxidants to reduce the production of free radicals is an important method to address the consequences of ischemia-reperfusion injury. Resveratrol is a natural active polyphenol compound with anti-inflammatory, antitumor, strong antioxidant and anti-free radical properties. The aim of this study was to investigate whether resveratrol could improve the effect of autologous ovarian transplantation after cryopreserve-thawn mouse ovarian tissue. Methods Whole-ovary vitrification and autotransplantation models were used to investigate the effects of resveratrol. Six-week-old female mice from the Institute of Cancer Research (ICR) were subjected to vitrification. All ovaries were preserved in liquid nitrogen for 1 week before being thawed. After thawing, ovarian tissues were autotransplanted in the bilateral kidney capsules. Mice ( n = 72) were randomly divided into four groups to determine the optimal concentration of resveratrol (experiment I). Treatments were given as follows: saline, 5 mg/kg resveratrol, 15 mg/kg resveratrol and 45 mg/kg resveratrol, which were administered orally for one week. Grafted ovaries were collected for analysis on days 3, 7, and 21 after transplantation. Ovarian follicle morphology was assessed by hematoxylin and eosin staining. Serum FSH and E 2 levels were measured to estimate the tr...