Puerarin prevents bone loss in ovariectomized mice and inhibits osteoclast formation in vitro

作者：Yuan, Siyuan, Sheng, Tong Cheng, Liu, Lian-Qi, Zhang, Yun-Ling, Xuemei, Ma -, Tao Tao, Zheng, Hồng, Yan, Ishimi, Yoshiko, Wang, Xin-Xiang · 发表于：中国天然药物 · 年份：2016 · 被引用次数：9 · 研究领域：Bone Metabolism and Diseases、Bone health and osteoporosis research、Bone health and treatments

The present study aimed at investigating the effects of Puerarin（PR）, a major isoflavonoid isolated from the Chinese medicinal herb Puerariae radix, on bone metabolism and the underlying mechanism of action. The in vivo assay, female mice were ovariectomized（OVX）, and the OVX mice were fed with a diet containing low, middle, and high doses of PR(2, 4, and 8 mg·d-1, respectively) or 17β-estradiol(E2, 0.03 μg·d-1) for 4 weeks. In OVX mice, the uterine weight declined, and intake of PR at any dose did not affect uterine weight, compared with the control. The total femoral bone mineral density（BMD） was significantly reduced by OVX, which was reversed by intake of the diet with PR at any dose, especially at the low dose. In the in vitro assay, RAW264.7 cells were used for studying the direct effect of PR on the formation of osteoclasts. PR reduced the formation of tartrate resistant acid phosphatase（TRAP）-positive multi-nucleated cells in the RAW 264.7 cells induced by receptor activator for nuclear factor-κB Ligand（RANKL）. MC3T3-E1 cells were used for studying the effects of PR on the expression of osteoprotegerin（OPG） and RANKL m RNA expression in osteoblasts. The expression of OPG m RNA and RANKL m RNA was detected by RT-PCR on Days of 5, 7, 10, and 12 after PR exposure. PR time-dependently enhanced the expression of OPG m RNA and reduced the expression of RANKL m RNA in MC3T3-E1 cells. In conclusion, our results suggest that PR can effectively prevent bone loss in OVX mice wit...