Two decades of one health surveillance of Nipah virus in Thailand

作者：Supaporn Wacharapluesadee, Siriporn Ghai, Prateep Duengkae, Pattarapol Maneeorn, Weerapong Thanapongtharm, Abhinbhen W. Saraya, Sangchai Yingsakmongkon, Yutthana Joyjinda, Sanipa Suradhat, Weenassarin Ampoot, Bundit Nuansrichay, Thongchai Kaewpom, Rachod Tantilertcharoen, Apaporn Rodpan, Kachen Wongsathapornchai, Teerada Ponpinit, Rome Buathong, Saowalak Bunprakob, Sudarat Damrongwatanapokin, Chanida Ruchiseesarod, Sininat Petcharat, Wantanee Kalpravidh, Kevin J. Olival, Martha M. Stokes, Thiravat Hemachudha · 发表于：One Health Outlook · 年份：2021 · DOI：10.1186/s42522-021-00044-9 · 被引用次数：20 · 研究领域：Virology and Viral Diseases、Immune responses and vaccinations、Mosquito-borne diseases and control

BACKGROUND: Nipah virus (NiV) infection causes encephalitis and has > 75% mortality rate, making it a WHO priority pathogen due to its pandemic potential. There have been NiV outbreak(s) in Malaysia, India, Bangladesh, and southern Philippines. NiV naturally circulates among fruit bats of the genus Pteropus and has been detected widely across Southeast and South Asia. Both Malaysian and Bangladeshi NiV strains have been found in fruit bats in Thailand. This study summarizes 20 years of pre-emptive One Health surveillance of NiV in Thailand, including triangulated surveillance of bats, and humans and pigs in the vicinity of roosts inhabited by NiV-infected bats. METHODS: Samples were collected periodically and tested for NiV from bats, pigs and healthy human volunteers from Wat Luang village, Chonburi province, home to the biggest P. lylei roosts in Thailand, and other provinces since 2001. Archived cerebrospinal fluid specimens from encephalitis patients between 2001 and 2012 were also tested for NiV. NiV RNA was detected using nested reverse transcription polymerase chain reaction (RT-PCR). NiV antibodies were detected using enzyme-linked immunosorbent assay or multiplex microsphere immunoassay. RESULTS: NiV RNA (mainly Bangladesh strain) was detected every year in fruit bats by RT-PCR from 2002 to 2020. The whole genome sequence of NiV directly sequenced from bat urine in 2017 shared 99.17% identity to NiV from a Bangladeshi patient in 2004. No NiV-specific IgG antibodies o...