Effect of NLRP3/GSDMD/IL-1β pathway in post epileptic depressive behavior of developmental rats and its possible intervention approaches

作者：Wen-Jie Zhao, Yang Chen, Wei Han, Jing Yang, Hong Sun, Dishu Huang, Yanan Pan, Heng-sheng Chen, Li Cheng, Li Jiang · 发表于：Jiefangjun yixue zazhi · 年份：2020 · 被引用次数：1 · 研究领域：Anesthesia and Neurotoxicity Research、Metabolism and Genetic Disorders、Neonatal and fetal brain pathology

Objective　To investigate the effect of NLRP3/GSDMD/IL-1β pathway in post epileptic depressive behavior of developmental rats and its possible intervention approaches. Methods　Sixty SD male rats aged 21 days were randomly divided into negative Control group, status epilepticus (SE) group, and SE+Dexamethasone (SE+DEX) group (20 each). The temporal lobe epilepsy (TLE) model was established by intraperitoneal injection of Lithium chloride-Pilocarpine (Licl-Pilocarpine) in SE group and SE+DEX group, the SE+DEX group was intraperitoneally injected with DEX [3 mg/(kg·d)] after the convulsion ceased for 7 consecutive days. Rats in SE group and Control group were given the same amount of normal saline at the same time point and by the same way. The post epileptic depressive behavior of rats was evaluated by sucrose partiality test (SPT), open field test (OPT) and forced swimming test (FST, n=8). Western blotting and RT-qPCR were used to detect the expression levels of protein and mRNA of NLRP3 and GSDMD in the hippocampus (n=3), and the expression level of IL-1β in the hippocampus was detected by ELISA (n=3). Results　Compared with control group, the sucrose consumption rate decreased (68.50%±8.65% vs. 87.13%±3.31%), the time spent in the central area of the open field was reduced [(1.25±0.55) s vs. (4.73±2.57) s], the total movement distance decreased [(7.34±1.48) cm vs. (11.01±1.94) cm], while the immobility time (IMT) in water increased [(53.74±22.54) s vs. (27.18±11.86) s] in SE g...