Mitochondria-Anchored Molecular Thermometer Quantitatively Monitoring Cellular Inflammations

作者：Zhenlong Huang, Ning Li, Xinfu Zhang, Yi Xiao · 发表于：Analytical Chemistry · 年份：2021 · DOI：10.1021/acs.analchem.0c04547 · 被引用次数：61 · 研究领域：ATP Synthase and ATPases Research、Mitochondrial Function and Pathology、Molecular Sensors and Ion Detection

Temperature in mitochondria can be a critical indicator of cell metabolism. Given the highly dynamic and inhomogeneous nature of mitochondria, it remains a big challenge to quantitatively monitor the local temperature changes during different cellular processes. To implement this task, we extend our strategy on mitochondria-anchored thermometers from “on–off” probe Mito-TEM to a ratiometric probe Mito-TEM 2.0 based on the Förster resonance energy transfer mechanism. Mito-TEM 2.0 exhibits not only a sensitive response to temperature through the ratiometric changes of dual emissions but also the specific immobilization in mitochondria via covalent bonds. Both characters support accurate and reliable detection of local temperature for a long time, even in malfunctioning mitochondria. By applying Mito-TEM 2.0 in fluorescence ratiometric imaging of cells and zebrafishes, we make a breakthrough in the quantitative visualization of mitochondrial temperature rises in different inflammation states.