Fine-Tuning Roles of Osa-miR159a in Rice Immunity Against Magnaporthe oryzae and Development

作者：Jinfeng Chen, Zhi‐Xue Zhao, Yan Li, Tingting Li, Yong Zhu, Xue‐Mei Yang, Shixin Zhou, He Wang, Ji‐Qun Zhao, Mei Pu, Hui Feng, Jing Fan, Ji‐Wei Zhang, Yanyan Huang, Wen-Ming Wang · 发表于：Rice · 年份：2021 · DOI：10.1186/s12284-021-00469-w · 被引用次数：50 · 研究领域：Plant Reproductive Biology、Fungal and yeast genetics research、Plant-Microbe Interactions and Immunity

BACKGROUND: Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice. An increasing number of microRNAs (miRNAs) have been reported to fine-tune rice immunity against M. oryzae and coordinate with growth and development. RESULTS: Here, we showed that rice microRNA159a (Osa-miR159a) played a positive role in rice resistance to M. oryzae. The expression of Osa-miR159a was suppressed in a susceptible accession at 12, 24, and 48 h post-inoculation (hpi); it was upregulated in a resistant accession of M. oryzae at 24 hpi. The transgenic rice lines overexpressing Osa-miR159a were highly resistant to M. oryzae. In contrast, the transgenic lines expressing a short tandem target mimic (STTM) to block Osa-miR159a showed enhanced susceptibility. Knockout mutations of the target genes of Osa-miR159a, including OsGAMYB, OsGAMYBL, and OsZF, led to resistance to M. oryzae. Alteration of the expression of Osa-miR159a impacted yield traits including pollen and grain development. CONCLUSIONS: Our results indicated that Osa-miR159a positively regulated rice immunity against M. oryzae by downregulating its target genes. Proper expression of Osa-miR159a was critical for coordinating rice blast resistance with grain development.