Proteasome maturation factor UMP1 confers broad-spectrum disease resistance by modulating H 2 O 2 accumulation in rice

作者：Xiao‐Hong Hu, Jing Fan, Jin-Long Wu, Shuai Shen, Jiaxue He, Jie Liu, He Wang, Yong Zhu, Guo‐Bang Li, Jing‐Hao Zhao, Jie Xu, Deqiang Li, Mei Pu, Zhi‐Xue Zhao, Shixin Zhou, Ji‐Wei Zhang, Yanyan Huang, Yàn Li, Yanli Lu, Fu Huang, Wenming Wang · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2021 · DOI：10.1101/2021.03.03.433750 · 被引用次数：1 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Pathogenic Bacteria Studies、Phytoplasmas and Hemiptera pathogens

ABSTRACT Crops with broad-spectrum resistance (BSR) to diseases are highly desirable in agricultural production. Identification of BSR loci and dissection of the underlying mechanisms are fundamental for crop resistance breeding. Here, we describe the identification and characterization of a rice UMP1 allele, which confers race-nonspecific BSR against blast pathogen Magnaporthe oryzae . OsUMP1 encodes a proteasome maturation factor that contributes to 26S proteasome abundance and activity in rice. Modulation of OsUMP1 expression leads to proteome changes, particularly affects the amounts and activities of H 2 O 2 -degrading enzymes. Consequently, H 2 O 2 accumulation and disease resistance are enhanced in OsUMP1- overexpressing rice but reduced in loss-of-function mutants. Elevation of OsUMP1 expression also promotes rice resistance to foliar pathogens Rhizoctonia solani and Xanthomonas oryzae pv. oryzae and a floral pathogen Ustilaginoidea virens without observable yield penalty. These results indicate a BSR pathway linking the proteasome machinery and H 2 O 2 homeostasis, and provide a candidate gene for balancing BSR and yield traits in rice breeding. One Sentence Summary A natural allele of rice UMP1 promotes resistance to multiple pathogens by boosting H 2 O 2 accumulation.