Identification and characterization of miRNAs and target genes response to hypoosmotic adaption in the cobia, Rachycentron canadum

作者：Baosong Huang, Fubai Liu, Danyu Cao, Gui-Ying Li, Kaiqi Lin, Jinfeng Li, Gang Shi, Jiandong Zhang, Bei Wang, Jiansheng Huang, Chuanhao Pan, Qian Ma, Gang Chen, Zhongliang Wang · 发表于：Aquaculture Research · 年份：2021 · DOI：10.1111/are.15177 · 被引用次数：5 · 研究领域：Aquaculture Nutrition and Growth、Physiological and biochemical adaptations、Marine Bivalve and Aquaculture Studies

Environmental salinity is a major factor influencing growth and development, reproduction, and physiology in fish. Recently, miRNAs have been confirmed to play an important role in regulating organismal adaptation in salinity challenge. Since the genomic studies on cobia (Rachycentron canadum) are limited, the miRNA transcriptome of R. canadum is important to investigate the salinity adaptation. In this study, the miRNA transcriptome analysis of the gills in R. canadum was performed under conditions of normal salinity (30‰) and low salinity (10‰). In total, 409 known miRNAs and 139 novel miRNAs were identified, with 56 DEMs (differentially expressed miRNAs). There were 64,983 target genes predicted based on the R. canadum transcriptome, in which 43,283 target genes were attributed to DEMs. The GO annotation and KEGG pathway analysis of target genes indicated that metabolic pathways and signal transduction were the most strongly affected pathways in R. canadum to tackle low-salinity stress, and R. canadum maintains osmotic homoeostasis and energy balance by regulating miRNAs in metabolism, ion channels, and transporters to adapt to low salinity. Overall, this study provides valuable genomic resources to further understanding the fundamental mechanisms of miRNA regulation in marine teleost response to hypoosmotic stress adaption.