Evaluation of the inhibitory effects of drugs on the growth of Babesia gibsoni using relative quantification real-time PCR

作者：Wenfang He, X X Feng, Xinxin Wu, Xiaohu Zhai, Y Y Li, B Zhang, Dongwei Yao · 发表于：Tropical biomedicine · 年份：2020 · DOI：10.47665/tb.37.4.871 · 被引用次数：2 · 研究领域：Vector-borne infectious diseases、Plant Virus Research Studies、Plant and Fungal Interactions Research

To evaluate the inhibitory effects of drugs on the growth of Babesia gibsoni, relative quantification real-time PCR method was developed in this study. The 18S rRNA gene was used as a target gene for the 2 -Ct method analysis. Additionally, chicken RNA was added to the parasitized blood before total RNA extraction. The chicken -actin gene was selected as an internal control gene for the 2 -Ct method analysis. The 100 L parasitized blood samples with different percentages of parasitized erythrocytes (PPEs) (3%, 1.5%, 0.75%, 0.375% and 0.1875%) were prepared for relative quantification of B. gibsoni. Regression analysis results revealed significant linear relationships between the relative quantification value and parasitemia. 18S rRNA gene expression was significantly decreased after treatment with diminazene aceturate and artesunate in vitro drug sensitivity test. This result suggested that this relative quantification real-time PCR method can be used to evaluate the effects of drug inhibition.