Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry

作者：Ioannis Skordos, Annelies Demeyer, Rudi Beyaert · 发表于：STAR Protocols · 年份：2021 · DOI：10.1016/j.xpro.2021.100351 · 被引用次数：36 · 研究领域：Immune Cell Function and Interaction、T-cell and B-cell Immunology、CAR-T cell therapy research

T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer. Monitoring of T cell frequency and function in genetically modified mice or murine models of disease is therefore of high interest. Here, we provide a detailed protocol to analyze regulatory T cells, T cell activation, and cytokine production in thymus, spleen, or blood via flow cytometry. For complete details on the use and execution of this protocol, please refer to Demeyer et al. (2020).