Integrated Analysis of Key Genes and Pathways Involved in Nonalcoholic Steatohepatitis Improvement After Roux-en-Y Gastric Bypass Surgery

作者：Chen Fu, Yong Zhou, Z. Wu, Yunze Li, Wenlong Zhou, Yong Wang · 发表于：Frontiers in Endocrinology · 年份：2021 · DOI：10.3389/fendo.2020.611213 · 被引用次数：17 · 研究领域：Liver Disease Diagnosis and Treatment、Ferroptosis and cancer prognosis、Bariatric Surgery and Outcomes

Background As the incidence of nonalcoholic fatty liver disease (NAFLD) increases globally, nonalcoholic steatohepatitis (NASH) has become the second common cause of liver transplantation for liver diseases. Recent evidence shows that Roux-en-Y gastric bypass (RYGB) surgery obviously alleviates NASH. However, the mechanism underlying RYGB induced NASH improvement is still elusive. Methods We obtained datasets, including hepatic gene expression data and histologic NASH status, at baseline and 1 year after RYGB surgery. Differentially expressed genes (DEGs) were identified comparing gene expression before and after RYGB surgery in each dataset. Common DEGs were obtained between both datasets and further subjected to functional and pathway enrichment analysis. Protein–protein interaction (PPI) network was constructed, and key modules and hub genes were also identified. Results In the present study, GSE106737 and GSE83452 datasets were included. One hundred thirty common DEGs (29 up-regulated and 101 down-regulated) were identified between GSE106737 and GSE83452 datasets. KEGG analysis showed that mineral absorption, IL-17 signaling pathway, osteoclast differentiation, and TNF signaling pathway were significantly enriched. Based on the PPI network, IGF1 , JUN , FOS , LDLR , TYROBP , DUSP1 , CXCR4 , ATF3 , CXCL2 , EGR1 , SAA1 , CTSS , and PPARA were identified as hub genes, and three functional modules were also extracted. Conclusion This study identifies the global gene expressio...