Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation

作者：Jianjia Li, Zhifa Wang, Xiangyu Huang, Zhaodan Wang, Zehao Chen, Runting Wang, Zhao Chen, Wei Liu, Buling Wu, Fuchun Fang, Wei Qiu · 发表于：Stem Cell Research & Therapy · 年份：2021 · DOI：10.1186/s13287-020-02123-6 · 被引用次数：33 · 研究领域：Sirtuins and Resveratrol in Medicine、Periodontal Regeneration and Treatments、Oral microbiology and periodontitis research

BACKGROUND: Human periodontal ligament stem cells (hPDLSCs) are ideal seed cells for periodontal regeneration. A greater understanding of the dynamic protein profiles during osteogenic differentiation contributed to the improvement of periodontal regeneration tissue engineering. METHODS: Tandem Mass Tag quantitative proteomics was utilized to reveal the temporal protein expression pattern during osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) were clustered and functional annotated by Gene Ontology (GO) terms. Pathway enrichment analysis was performed based on the Kyoto Encyclopedia of Genes and Genomes database, followed by the predicted activation using Ingenuity Pathway Analysis software. Interaction networks of redox-sensitive signalling pathways and oxidative phosphorylation (OXPHOS) were conducted and the hub protein SOD2 was validated with western blotting. RESULTS: A total of 1024 DEPs were identified and clustered in 5 distinctive clusters representing dynamic tendencies. The GO enrichment results indicated that proteins with different tendencies show different functions. Pathway enrichment analysis found that OXPHOS was significantly involved, which further predicted continuous activation. Redox-sensitive signalling pathways with dynamic activation status showed associations with OXPHOS to various degrees, especially the sirtuin signalling pathway. SOD2, an important component of the sirtuin pathway, displays a ...