LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis

作者：Dan Xu, Qingxia Song, Ying Liu, Wansu Chen, Lijuan Lu, Min Xu, Xiaohui Fang, Wenjie Zhao, Huifang Zhou · 发表于：Journal of Cancer · 年份：2021 · DOI：10.7150/jca.51457 · 被引用次数：31 · 研究领域：Cancer-related molecular mechanisms research、Circular RNAs in diseases、MicroRNA in disease regulation

Objective: To clarify the role of LINC00665 in ovarian cancer (OC) progression and the possible mechanism. Methods: LINC00665 levels in OC tissues and cell lines were detected by qRT-PCR. The correlation between LINC00665 and clinicopathologic characteristics of OC patients was assessed. Biological functions of OC cell phenotypes influenced by LINC00665 were examined by CCK-8, colony formation and Transwell assay. Dual-luciferase reporter assay and RIP assay were conducted to verify the interaction between LINC00665 and its downstream target. Results: LINC00665 was upregulated in OC and linked to poor prognosis. Knockdown of LINC00665 blocked malignant proliferative, migratory and invasive functions of OC cells. By competitively binding miRNA-34a-5p, LINC00665 abolished the inhibitory effect of miR-34a-3p on its downstream gene E2F3, thus promoting OC progression. Conclusion: LINC00665/miRNA-34a-5p/E2F3 axis is involved in OC progression, providing novel insights into the clinical treatment of OC.