A native, highly active Tc1/mariner transposon from zebrafish ( ZB ) offers an efficient genetic manipulation tool for vertebrates

作者：Dan Shen, Chengyi Song, Csaba Miskey, Shuheng Chan, Zhongxia Guan, Yatong Sang, Yali Wang, Cai Chen, Xiaoyan Wang, Ferenc Müller, Zoltán Ivics, Bo Gao · 发表于：Nucleic Acids Research · 年份：2021 · DOI：10.1093/nar/gkab045 · 被引用次数：29 · 研究领域：Chromosomal and Genetic Variations、CRISPR and Genetic Engineering、Advanced biosensing and bioanalysis techniques

New genetic tools and strategies are currently under development to facilitate functional genomics analyses. Here, we describe an active member of the Tc1/mariner transposon superfamily, named ZB, which invaded the zebrafish genome very recently. ZB exhibits high activity in vertebrate cells, in the range of those of the widely used transposons piggyBac (PB), Sleeping Beauty (SB) and Tol2. ZB has a similar structural organization and target site sequence preference to SB, but a different integration profile with respect to genome-wide preference among mammalian functional annotation features. Namely, ZB displays a preference for integration into transcriptional regulatory regions of genes. Accordingly, we demonstrate the utility of ZB for enhancer trapping in zebrafish embryos and in the mouse germline. These results indicate that ZB may be a powerful tool for genetic manipulation in vertebrate model species.