A novel intramural TGF β 1 hydrogel delivery method to decrease murine abdominal aortic aneurysm and rat aortic pseudoaneurysm formation and progression

作者：Hualong Bai, Peng Sun, Shunbo Wei, Boao Xie, Mingxing Li, Mingxing Li, Yanhua Xu, Wang Wang, Wang Wang, Yuanfeng Liu, Liwei Zhang, Haoliang Wu, Zhiju Wang, Zhiju Wang, Ying Xing, Zhiwei Wang, Zhiwei Wang, Jingan Li · 发表于：Biomedicine & Pharmacotherapy · 年份：2021 · DOI：10.1016/j.biopha.2021.111296 · 被引用次数：22 · 研究领域：Aortic aneurysm repair treatments、Tissue Engineering and Regenerative Medicine、Infectious Aortic and Vascular Conditions

Objectives Aneurysms are generally the result of dilation of all 3 layers of the vessel wall, and pseudoaneurysms are the result of localized extravasation of blood that is contained by surrounding tissue. Since there is still no recommended protocol to decrease aneurysm formation and progression, we hypothesised that intramural delivery of TGF β1 hydrogel can decrease aneurysm and pseudoaneurysm formation and progression. Materials Male C57BL/6 J mice (12–14 wk), SD rats (200 g) and pig abdominal aortas were used, and hydrogels were fabricated by the interaction of sodium alginate (SA), hyaluronic acid (HA) and CaCO 3 . Methods A CaCl 2 adventitial incubation model in mice and a decellularized human great saphenous vein patch angioplasty model in rats were used. TGF β1 hydrogel was intramurally delivered after CaCl 2 incubation in mice; at day 7, the abdomen in some mice was reopened, and TGF β1 hydrogel was injected intramurally into the aorta. In rats, TGF β1 hydrogel was delivered intramurally after patch angioplasty completion. Tissues were harvested at day 14 and analysed by histology and immunohistochemistry staining. The pig aorta was also intramurally injected with hydrogel. Results In mice, rhodamine hydrogel was still found between the medium and adventitia at day 14. In the mouse aneurysm model, there was a thicker wall and smaller amount of elastin breaks in the TGF β1 hydrogel-delivered groups both at day 0 and day 7 after CaCl 2 incubation, and there were large...