Conjugating Peptides onto 1D Rodlike Bionanoparticles for Enhanced Activity against Gram-Negative Bacteria

作者：Guocheng Xie, Sijia Gao, Jinzhao Ou, Meng Zhu, Man Wu, Xiaoyan Ju, Zhuang Li, Ye Tian, Zhongwei Niu · 发表于：Nano Letters · 年份：2021 · DOI：10.1021/acs.nanolett.0c04516 · 被引用次数：25 · 研究领域：Antimicrobial Peptides and Activities、Bacteriophages and microbial interactions、Respiratory viral infections research

Gram-negative bacteria, which possess an impermeable outer membrane, are responsible for many untreatable infections. The lack of development of new relevant antibiotics for over 50 years has increased threats. Peptides are regarded as the most promising alternatives to antibiotics. However, since the activities of existing peptides are not yet comparable to those of current antibiotics, there is an urgent need to improve their antibacterial efficiencies. Herein, we conjugate peptides onto one-dimensional rod-like tobacco mosaic virus (TMV). The peptides on the obtained nanoparticles (peptide–TMV) are hundreds of times superior to free peptides in combating Gram-negative bacteria. Through morphology and gene detection of Escherichia coli, it was revealed that following peptide–TMV application, the high osmotic pressure related to membrane damage and the generated reactive oxygen species cause Escherichia coli ’s death. In addition, peptide–TMV causes a downregulation of biofilm-related genes, inhibiting biofilm formation. This work paves the way to combat Gram-negative bacteria-related infection.