Characteristics ofSalmonellaRecovered From Stools of Children Enrolled in the Global Enteric Multicenter Study

作者：Irene N Kasumba, Caisey V. Pulford, Blanca M. Perez-Sepulveda, Sunil Kumar Sen, Nurulla Sayed, Jasnehta Permala-Booth, Sofie Livio, Darren Heavens, Ross Low, Neil Hall, Anna Roose, Helen Powell, Tamer H. Farag, Sandra Panchalingham, Lynette Y. Berkeley, Dilruba Nasrin, William C. Blackwelder, Yukun Wu, Boubou Tamboura, Doh Sanogo, Uma Onwuchekwa, Samba O. Sow, John B. Ochieng, Richard Omore, Joseph Oundo, Robert F. Breiman, Eric D. Mintz, Ciara E. O’Reilly, Martín Antonio, Debasish Saha, M Jahangir Hossain, Inácio Mandomando, Quique Bassat, Pedro L. Alonso, T. Ramamurthy, Dipika Sur, Shahida Qureshi, Anita K. M. Zaidi, Anowar Hossain, Abu Syed Golam Faruque, James P. Nataro, Karen L. Kotloff, Myron M. Levine, Jay C. D. Hinton, Sharon M. Tennant · 发表于：Clinical Infectious Diseases · 年份：2021 · DOI：10.1093/cid/ciab051 · 被引用次数：27 · 研究领域：Salmonella and Campylobacter epidemiology、Escherichia coli research studies、Listeria monocytogenes in Food Safety

BACKGROUND: The Global Enteric Multicenter Study (GEMS) determined the etiologic agents of moderate-to-severe diarrhea (MSD) in children under 5 years old in Africa and Asia. Here, we describe the prevalence and antimicrobial susceptibility of nontyphoidal Salmonella (NTS) serovars in GEMS and examine the phylogenetics of Salmonella Typhimurium ST313 isolates. METHODS: Salmonella isolated from children with MSD or diarrhea-free controls were identified by classical clinical microbiology and serotyped using antisera and/or whole-genome sequence data. We evaluated antimicrobial susceptibility using the Kirby-Bauer disk-diffusion method. Salmonella Typhimurium sequence types were determined using multi-locus sequence typing, and whole-genome sequencing was performed to assess the phylogeny of ST313. RESULTS: Of 370 Salmonella-positive individuals, 190 (51.4%) were MSD cases and 180 (48.6%) were diarrhea-free controls. The most frequent Salmonella serovars identified were Salmonella Typhimurium, serogroup O:8 (C2-C3), serogroup O:6,7 (C1), Salmonella Paratyphi B Java, and serogroup O:4 (B). The prevalence of NTS was low but similar across sites, regardless of age, and was similar among both cases and controls except in Kenya, where Salmonella Typhimurium was more commonly associated with cases than controls. Phylogenetic analysis showed that these Salmonella Typhimurium isolates, all ST313, were highly genetically related to isolates from controls. Generally, Salmonella isolates ...