Evaluation of aromatic amino acids as potential biomarkers in breast cancer by Raman spectroscopy analysis

作者：Shaymus Contorno, Richard Darienzo, Rina Tannenbaum · 发表于：Scientific Reports · 年份：2021 · DOI：10.1038/s41598-021-81296-3 · 被引用次数：70 · 研究领域：Spectroscopy Techniques in Biomedical and Chemical Research、Spectroscopy and Chemometric Analyses、Identification and Quantification in Food

Abstract The scope of the work undertaken in this paper was to explore the feasibility and reliability of using the Raman signature of aromatic amino acids as a marker in the detection of the presence of breast cancer and perhaps, even the prediction of cancer development in very early stages of cancer onset. To be able to assess this hypothesis, we collected most recent and relevant literature in which Raman spectroscopy was used as an analytical tool in the evaluation of breast cell lines and breast tissue, re-analyzed all the Raman spectra, and extracted all spectral bands from each spectrum that were indicative of aromatic amino acids. The criteria for the consideration of the various papers for this study, and hence, the inclusion of the data that they contained were two-fold: (1) The papers had to focus on the characterization of breast tissue with Raman spectroscopy, and (2) the spectra provided within these papers included the spectral range of 500–1200 cm −1 , which constitutes the characteristic region for aromatic amino acid vibrational modes. After all the papers that satisfied these criteria were collected, the relevant spectra from each paper were extracted, processed, normalized. All data were then plotted without bias in order to decide whether there is a pattern that can shed light on a possible diagnostic classification. Remarkably, we have been able to demonstrate that cancerous breast tissues and cells decidedly exhibit overexpression of aromatic amino aci...