Biomimetic Keratin Gold Nanoparticle-Mediated In Vitro Photothermal Therapy on Glioblastoma Multiforme

作者：Alexa Guglielmelli, Paolo Rosa, Marco Contardi, Mirko Prato, Giorgio Mangino, Selenia Miglietta, Vincenzo Petrozza, Roberto Pani, Antonella Calogero, Athanassia Athanassiou, Giovanni Perotto, Luciano De Sio · 发表于：Nanomedicine · 年份：2021 · DOI：10.2217/nnm-2020-0349 · 被引用次数：50 · 研究领域：Gold and Silver Nanoparticles Synthesis and Applications、Nanoparticles: synthesis and applications、Nanoplatforms for cancer theranostics

Aim: To realize and characterize a new generation of keratin-coated gold nanoparticles (Ker-AuNPs) as highly efficient photosensitive nanosized therapeutics for plasmonic photothermal (PPT) therapy. Materials & methods: The chemical, physical, morphological and photothermal properties of Ker-AuNPs are investigated using dynamic light scattering, ζ-potential, UV–Visible, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, transmission electron microscopy and high-resolution thermography. In vitro experiments are performed on a human glioblastoma cell line (i.e., U87-MG), using viability assays, transmission electron microscopy, fluorescence microscopy, cytometric analyses and PPT experiments. Results: Experiments confirm the excellent biocompatibility of Ker-AuNPs, their efficient cellular uptake and localized photothermal heating capabilities. Conclusion: The reported structural and functional properties pointed out these Ker-AuNPs as a promising new tool in the field of biocompatible photothermal agents for PPT treatments against cancer-related diseases.