Best practices in plant cytometry

作者：David W. Galbraith, João Loureiro, Ioanna Antoniadi, Jillian D. Bainard, Petr Bureš, Petr Cápal, Mariana Castro, Sílvia Castro, Martin Čertner, Dora Čertnerová, Zuzana Chumová, Jaroslav Doležel, Debora Giorgi, Brian C. Husband, Filip Kolář, Petr Koutecký, Paul Kron, Ilia J. Leitch, Karin Ljung, Sara Lopes, Magdalena Lučanová, Sergio Lucretti, Wen Ma, Susanne Melzer, István Molnár, Ondřej Novák, Nicole Poulton, Vladimír Skalický, Elwira Śliwińska, Petr Šmarda, Tyler Smith, Guiling Sun, Pedro Talhinhas, Attila Tárnok, Eva M. Temsch, Pavel Trávníček, Tomáš Urfus · 发表于：Cytometry Part A · 年份：2021 · DOI：10.1002/cyto.a.24295 · 被引用次数：34 · 研究领域：Chromosomal and Genetic Variations、Plant Pathogens and Resistance、Single-cell and spatial transcriptomics

Flow cytometry (FCM) and flow cytometric sorting (FCS) systems have developed as experimental tools of remarkable power and are enjoying an ever-increasing impact in the general field of biology.1 Application of these tools to plant biology has developed more slowly given that the natural form of plants infrequently resembles that of the single cell suspension, prototypically the hematopoietic system that drove the original development of FCM/FCS. Nevertheless, these systems have had a profound influence at all levels of plant biology, from the study of single cells and subcellular organelles, to the behavior of populations of plants, and ultimately to the performance of ecosystems. It is safe to say their impact has not plateaued, as further applications of this unique technology are increasingly developed by innovative scientists around the world to address questions both in the basic sciences, and to increasingly confront emerging problems in the applied sector. For example, in addressing the challenges of sustainable production of sufficient food resources based on plant breeding involving ploidy-based approaches (e.g., induction of polyploidy)2 for the needs of our future global citizens, FCM, and FCS systems will play central roles in this effort. The degree to which FCM and FCS systems have impacted plant biology and applied agricultural sciences must not be understated. The major applications of DNA FCM are ploidy level and genome size estimations, and cell cycle anal...