GC-1 spg cells are most similar to Leydig cells, a testis somatic cell-type, and not germ cells

作者：Andrew R. Norman, Lauren Byrnes, Jeremy F. Reiter · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2021 · DOI：10.1101/2021.01.07.425754 · 被引用次数：3 · 研究领域：Sperm and Testicular Function、RNA Interference and Gene Delivery、Animal Genetics and Reproduction

Abstract GC-1 spg is an immortalized cell line derived from an adult mouse testis and reported to be most similar to spermatocytes, a male germ cell-type. However, immunofluorescence indicates that GC-1 spg cells express WT1, a marker of testis somatic cells, and do not express markers of germ cells. Transcriptomic profiling indicate GC-1 cells are most similar to Leydig cells. Therefore, we conclude that GC-1 spg cells are most similar to testis somatic cells.