In vitro co-culture system for Prunus spp. and Armillaria mellea in phenolic foam rooting matric

作者：Jeffrey Adelberg, Jacqueline Naylor-Adelberg, Sarah Miller, Ksenija Gašić, Guido Schnabel, Patricia K. Bryson, Christopher Saski, Stephen Parris, G.L. Reighard · 发表于：In Vitro Cellular & Developmental Biology - Plant · 年份：2021 · DOI：10.1007/s11627-020-10136-2 · 被引用次数：17 · 研究领域：Plant Pathogens and Fungal Diseases、Plant Disease Management Techniques、Nematode management and characterization studies

Abstract Armillaria and Desarmillaria spp. are causal agents of a devastating root-borne disease of peach. Breeding resistant rootstock requires a reliable screening tool. An in vitro co-culture screen designed for almond was modified by replacing agar-gelled medium with a more aerated phenolic foam and combining resistant and susceptible rootstocks ( i.e. , common garden experiment) and minimizes variation in inoculum pressure or rooting substrate among replicate vessels. Eight Prunus rootstocks tested (peach, plum, peach × plum, and choke cherry) were rooted and had no decline in health. Susceptible peach rootstock, ‘GF 305’, was cultured for 15 wk in phenolic foam in the same vessel with a resistant peach × plum hybrid, ‘MP-29’, inoculated with Armillaria mellea at week 5, that led to more severe shoot symptoms in the former after an additional 8 wk. This method accommodated peach genotypes that were difficult to root in agar medium. The difference during a uniform challenge with the A. mellea fungus recapitulates resistant/susceptible reactions. The phenolic foam-based co-culture method will work on many Prunus spp . of potential use in rootstock breeding.