Physiological and Proteomic Signatures Reveal Mechanisms of Superior Drought Resilience in Pearl Millet Compared to Wheat

作者：Arindam Ghatak, Palak Chaturvedi, Gert Bachmann, Luís Valledor, Živa Ramšak, Mitra Mohammadi Bazargani, Prasad Bajaj, Sridharan Jegadeesan, Weimin Li, Xiaoliang Sun, Kristina Gruden, Rajeev K. Varshney, Wolfram Weckwerth · 发表于：Frontiers in Plant Science · 年份：2021 · DOI：10.3389/fpls.2020.600278 · 被引用次数：83 · 研究领域：Plant responses to water stress、Plant Stress Responses and Tolerance、Wheat and Barley Genetics and Pathology

Presently, pearl millet and wheat are belonging to highly important cereal crops. Pearl millet, however, is an under-utilized crop, despite its superior resilience to drought and heat stress in contrast to wheat. To investigate this in more detail, we performed comparative physiological screening and large scale proteomics of drought stress responses in drought-tolerant and susceptible genotypes of pearl millet and wheat. These chosen genotypes are widely used in breeding and farming practices. The physiological responses demonstrated large differences in the regulation of root morphology and photosynthetic machinery, revealing a stay-green phenotype in pearl millet. Subsequent tissue-specific proteome analysis of leaves, roots and seeds led to the identification of 12,558 proteins in pearl millet and wheat under well-watered and stress conditions. To allow for this comparative proteome analysis and to provide a platform for future functional proteomics studies we performed a systematic phylogenetic analysis of all orthologues in pearl millet, wheat, foxtail millet, sorghum, barley, brachypodium, rice, maize, Arabidopsis, and soybean. In summary, we define (i) a stay-green proteome signature in the drought-tolerant pearl millet phenotype and (ii) differential senescence proteome signatures in contrasting wheat phenotypes not capable of coping with similar drought stress. These different responses have a significant effect on yield and grain filling processes reflected by the ...