Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

作者：Gotaro Toda, Toshimasa Yamauchi, Takashi Kadowaki, Kohjiro Ueki · 发表于：STAR Protocols · 年份：2020 · DOI：10.1016/j.xpro.2020.100246 · 被引用次数：425 · 研究领域：Immune cells in cancer、Adipokines, Inflammation, and Metabolic Diseases、Extracellular vesicles in disease

The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).