Lnc-GULP1–2:1 affects granulosa cell proliferation by regulating COL3A1 expression and localization

作者：Guidong Yao, Yue Kong, Guang Yang, Deqi Kong, Yijiang Xu, Jiahuan He, Ziwen Xu, Yucheng Bai, Huiying Fan, Qina He, Yingpu Sun · 发表于：Journal of Ovarian Research · 年份：2021 · DOI：10.1186/s13048-021-00769-1 · 被引用次数：27 · 研究领域：Gynecological conditions and treatments、Reproductive Biology and Fertility、Cancer-related molecular mechanisms research

BACKGROUNDS: Long non-coding RNA is a novel group of non-protein coding transcripts over 200 nt in length. Recent studies have found that they are widely involved in many pathological and physiological processes. In our previous study, we found that lnc-GULP1-2:1 was significantly down-regulated in the ovarian cortical tissue of patients with primary ovarian insufficiency and predicted that lnc-GULP1-2:1 has a regulatory effect on COL3A1. RESULTS: In this study, we found that lnc-GULP1-2:1 was mainly localized in the cytoplasm of luteinized granulosa cells. The expression of lnc-GULP1-2:1 was lower in patients with diminished ovarian reserve but substantially elevated in patients with polycystic ovary syndrome. Overexpression of lnc-GULP1-2:1 in KGN cells significantly inhibited cell proliferation, likely through cell cycle related genes CCND2 and p16. Moreover, lnc-GULP1-2:1 expression was positively correlated with the level of COL3A in luteinized granulosa cells from patients with different ovarian functions as well as in multiple cell lines. Overexpression of lnc-GULP1-2:1 in KGN cells promoted the expression of COL3A1 and its translocation into the nucleus. Consistently, silencing COL3A1 in KGN cells also significantly inhibited cell proliferation. CONCLUSIONS: Lnc-GULP1-2:1 affects the proliferation of granulosa cells by regulating the expression and localization of COL3A1 protein, and may participate in the regulation of ovarian follicle development. This study will pr...