A Label-Free Proteomic Strategy to Investigate the Intramuscular fat Proteomic Differences Between Biceps Femoris and Longissimus Dorsi in Inner Mongolian Cashmere Goats

作者：Yuchun Xie, Zhihong Liu, Rile Nai, Juntao Guo, Xin Su, Cun Zhao, Chongyan Zhang, Qing Qin, Dongliang Dai, Zhixin Wang, Yanhong Zhao, Rui Su, Zhiying Wang, Ruijun Wang, Yanjun Zhang, Jinquan Li, Haijun Li · 发表于：Research Square · 年份：2020 · DOI：10.21203/rs.3.rs-131926/v1 · 被引用次数：1 · 研究领域：Meat and Animal Product Quality、Genetic and phenotypic traits in livestock、Cancer, Lipids, and Metabolism

Abstract Background: As a major raw-cashmere-producing province in China. Nearly 700,000 Aerbasi cashmere goats are fed per year, and the corresponding meat production is nearly 10,000 tons. However, there are no reports on the meat of this goat. To better understand the molecular variations underlying intramuscular fat (IMF) anabolism and catabolism in Inner Mongolian cashmere goats, the proteomic differences between the biceps femoris (BF) and longissimus dorsi (LD) were investigated by a label-free strategy. Then, the identified proteins were verified as being involved in IMF anabolism and catabolism by Western blot analysis. Results: The IMF content was significantly higher in the BF than in the LD, suggesting that IMF accumulated more in the BF or was metabolized more in the LD. We performed proteomic analysis of IMF anabolism and catabolism at the proteomic level, and 1209 proteins were identified in the BF (high-IMF) and LD (low-IMF) groups. Among them, 110 were differentially expressed proteins (DEPs), 81 of which were upregulated in the high-IMF group, while 29 were upregulated in the low-IMF group. Gene ontology (GO) classification showed that the 110 DEPs were functionally classified into 100 annotation clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the 110 DEPs covered 34 KEGG pathways. Three pathways were related to IMF metabolism and deposition—fatty acid metabolism, fatty acid degradation and fatty acid elongation—and included 7 p...