QTL mapping of root traits in wheat under different phosphorus levels using hydroponic culture

作者：Mengjiao Yang, Cairong Wang, Muhammad Adeel Hassan, Faji Li, Xianchun Xia, Shubing Shi, Yonggui Xiao, Zhonghu He · 发表于：BMC Genomics · 年份：2021 · DOI：10.1186/s12864-021-07425-4 · 被引用次数：40 · 研究领域：Plant nutrient uptake and metabolism、Wheat and Barley Genetics and Pathology、Rice Cultivation and Yield Improvement

BACKGROUND: Phosphorus (P) is an important in ensuring plant morphogenesis and grain quality, therefore an efficient root system is crucial for P-uptake. Identification of useful loci for root morphological and P uptake related traits at seedling stage is important for wheat breeding. The aims of this study were to evaluate phenotypic diversity of Yangmai 16/Zhongmai 895 derived doubled haploid (DH) population for root system architecture (RSA) and biomass related traits (BRT) in different P treatments at seedling stage using hydroponic culture, and to identify QTL using 660 K SNP array based high-density genetic map. RESULTS: All traits showed significant variations among the DH lines with high heritabilities (0.76 to 0.91) and high correlations (r = 0.59 to 0.98) among all traits. Inclusive composite interval mapping (ICIM) identified 34 QTL with 4.64-20.41% of the phenotypic variances individually, and the log of odds (LOD) values ranging from 2.59 to 10.43. Seven QTL clusters (C1 to C7) were mapped on chromosomes 3DL, 4BS, 4DS, 6BL, 7AS, 7AL and 7BL, cluster C5 on chromosome 7AS (AX-109955164 - AX-109445593) with pleiotropic effect played key role in modulating root length (RL), root tips number (RTN) and root surface area (ROSA) under low P condition, with the favorable allele from Zhongmai 895. CONCLUSIONS: This study carried out an imaging pipeline-based rapid phenotyping of RSA and BRT traits in hydroponic culture. It is an efficient approach for screening of large po...