Heterologous expression of Arabidopsis thaliana rty gene in strawberry (Fragaria × ananassa Duch.) improves drought tolerance

作者：Maofu Li, Yuan Yang, Ali Raza, Shanshan Yin, Hua Wang, Yuntao Zhang, Jing Dong, Guixia Wang, Chuanfei Zhong, Hong Zhang, Jiashen Liu, Wanmei Jin · 发表于：BMC Plant Biology · 年份：2021 · DOI：10.1186/s12870-021-02839-4 · 被引用次数：29 · 研究领域：Plant Stress Responses and Tolerance、Plant Molecular Biology Research、Plant Gene Expression Analysis

Abstract Background Strawberry ( Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY ( RTY ) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferase II ( NPT II). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g − 1 and 66.0 ng g − 1 in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g − 1 and in transgenic plants were 543.8 ng g − 1 . The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher ...