Comparison of proteomic profiles from the testicular tissue of males with impaired and normal spermatogenesis

作者：Jiaying Liang, Yichun Zheng, Weihong Zeng, Liuqing Chen, Shaofen Yang, Peng Du, Yujiang Wang, Xingsu Yu, Xiqian Zhang · 发表于：Systems Biology in Reproductive Medicine · 年份：2020 · DOI：10.1080/19396368.2020.1846822 · 被引用次数：54 · 研究领域：Sperm and Testicular Function、Hormonal and reproductive studies、Reproductive Biology and Fertility

In this study, we aimed to explore the potential differences in proteomic profiles from the testicular tissue of azoospermatic men with impaired spermatogenesis and normal spermatogenesis. Isobaric tags for relative and absolute quantitation (iTRAQ) labeled technology and LC-MS/MS technology were used to identify differentially expressed proteins. Potential functions of differentially expressed proteins were predicted using gene ontology (GO) and the Kyoto encyclopedia of genes and genomes (KEGG). Immunohistochemistry (IHC) and western blot (WB) were used to verify the differentially expressed proteins. A protein–protein interaction (PPI) network was built to outline the regulatory network of differentially expressed proteins. A total of 3,945 proteins were identified in men with normal and impaired spermatogenesis. Of these, 116 proteins were differentially expressed in men with impaired spermatogenesis: 39 were upregulated and 77 were downregulated. Furthermore, we found that these differentially expressed proteins were mainly involved in the cellular component, which may be mainly associated with the spliceosome, ribosome, and thyroid hormone synthesis signaling pathways. The spliceosome- and ribosome-associated proteins YBX1, FBL, and HNRNPU were downregulated. And the proteomic profile of testicular tissue in men with impaired spermatogenesis is different from that of men with normal spermatogenesis. For this reason, differentially expressed proteins such as YBX1, FBL an...