Cyclopentane FIT-PNAs: bright RNA sensors

作者：Odelia Tepper, Hongchao Zheng, Daniel H. Appella, Eylon Yavin · 发表于：Chemical Communications · 年份：2020 · DOI：10.1039/d0cc07400d · 被引用次数：18 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA and protein synthesis mechanisms、RNA Interference and Gene Delivery

Cyclopentane modified FIT-PNA (cpFIT-PNA) probes are reported as highly emissive RNA sensors with the highest reported brightness for FIT-PNAs. Compared to FIT-PNAs, cpFIT-PNAs have improved mismatch discrimination for several pyrimidine-pyrimidine single nucleotide variants (SNVs).