Light-Controlled Release of Therapeutic Proteins from Red Blood Cells

作者：Brianna M. Vickerman, Colin P. O’Banion, Xianming Tan, David S. Lawrence · 发表于：ACS Central Science · 年份：2020 · DOI：10.1021/acscentsci.0c01151 · 被引用次数：21 · 研究领域：Erythrocyte Function and Pathophysiology、Lipid Membrane Structure and Behavior、Hemoglobin structure and function

High Resolution Image Download MS PowerPoint Slide Protein therapeutics are a powerful class of drugs known for their selectivity and potency. However, the potential efficacy of these therapeutics is commonly offset by short circulatory half-lives and undesired action at otherwise healthy tissue. We describe herein a targeted protein delivery system that employs engineered red blood cells (RBCs) as carriers and light as the external trigger that promotes hemolysis and drug release. RBCs internally loaded with therapeutic proteins are readily surface modified with a dormant hemolytic peptide. The latter is activated via easily assigned wavelengths that extend into the optical window of tissue. We have demonstrated that photorelease transpires with spatiotemporal control and that the liberated proteins display the anticipated biological effects in vitro . Furthermore, we have confirmed targeted delivery of a clot-inducing enzyme in a mouse model. Finally, we anticipate that this strategy is not limited to RBC carriers but also should be applicable to nano- and microtransporters comprised of bilayer lipid membranes.