<p>Effect and Mechanism of <em>Ganoderma lucidum</em> Spores on Alleviation of Diabetic Cardiomyopathy in a Pilot in vivo Study</p>

作者：Fahmi Shaher, Shuqiu Wang, Hongbin Qiu, Yu Hu, Yu Zhang, Weiqun Wang, Hisham Al-Ward, Mahfoudh A. M. Abdulghani, Salem Baldi, Shaobo Zhou · 发表于：Diabetes Metabolic Syndrome and Obesity · 年份：2020 · DOI：10.2147/dmso.s281527 · 被引用次数：16 · 研究领域：Fungal Biology and Applications、Fungal and yeast genetics research、Polysaccharides and Plant Cell Walls

BACKGROUND: spores (GLS) exhibit disease prevention properties, but no study has been carried out on the anti-diabetic cardiomyopathy property of GLS. The aim of this study was to evaluate the hyperglycemia-mediated cardiomyopathy protection and mechanisms of GLS in streptozotocin (STZ)induced diabetic rats. METHODS: Male SD rats were randomly divided into three groups. Two groups were given STZ (50 mg/kg, i.p.) treatment and when their fasting plasma glucose was above 16.7 mmol/L, among them, one group was given placebo, as diabetic group, and another group was given GLS (300 mg/kg) treatment. The group without STZ treatment was given placebo as a control group. The experiment lasted 70 days. The histology of myocardium and biomarkers of antioxidants, myocardial injury, pro-inflammatory cytokines, pro-apoptotic proteins and phosphorylation of key proteins in PI3K/AKT pathway were assessed. RESULTS: Biochemical analysis showed that GLS treatment significantly reduced the blood glucose (-20.3%) and triglyceride (-20.4%) levels compared to diabetic group without treatment. GLS treatment decreased the content of MDA (-25.6%) and activity of lactate dehydrogenase (-18.9%) but increased the activity of GSH-Px (65.4%). Western blot analysis showed that GLS treatment reduced the expression of both alpha-smooth muscle actin and brain natriuretic peptide. Histological analysis on the cardiac tissue micrographs showed that GLS treatment reduced collagen fibrosis and glycogen reactivity...